CULTURES OF ALVEOLOCYTES I AND II TYPE FOR MEDICAL AND ECOLOGICAL RESEARCH

The alveolar epithelium is a dynamic tissue, consisting of cells types I and II, covering more than 99 % the lung inner surface actively responding to various endogenous exogenous stimuli. technology alveolocyte isolation presented, which consists in mechanical disaggregation tissue with subsequent processing resulting explants 0.25 trypsin solution combination filtration cell suspension through pores diameter 100 µm 50 µm. In two-dimensional static culture viable dividing rounded large epithelial cuboid or polygonal morphology, producing surfactant proteins, were visualized.